Homozygous mutant lines exhibit editing defects in accD C794. RNA assayed in each lane was prepared separately from a single leaf of a different individual plant. E, U, and O indicate edited extension product, unedited extension product, and oligonucleotide, respectively, and represent 34, 30, and 22 nt for the C794 site. (A) PPE of RNA from Columbia (wt), heterozygous, and homozygous WiscDsLox330H10 plants. The edited and unedited extension products and oligonucleotide for the C1568 site are 33, 31, and 24 nt in size. (B) PPE assay of accD C794 editing in progeny of a heterozygous plant carrying the FLAG_424E06 insertion allele. WT (ecotype WS-4), heterozygotes (+/−) and homozygotes (−/−). Control lanes g and o correspond to genomic DNA template and oligonucleotide only, respectively. (C) PPE assay of accD C794 editing in Columbia (WT), a heterozygote (+/−) and homozygous mutant (−/−) plants carrying the GABI_167A04 insertion allele.
