A two-step model for the release of Vg1 mRNA from the cortex in meiotic maturation. Initiating triggers for the phosphorylation of Vg1RBP (boxed) are indicated at the bottom (application of progesterone or microinjection of MKK6-DD) with the physiological pathway arranged temporally (from left to right; not to scale). The components contributing to Vg1 mRNA's subcortical anchoring at the key stages are represented in the main illustration (see key in the figure). The solubility of Vg1 mRNA in high or low salt buffers is indicated above. The activity of several inhibitors used throughout this study is in light gray. Direct links are in solid arrows and indirect connections are in dashed arrows. Phosph., phosphorylation; R, RRMs; K, KH didomains.
