Kinetics of P-bodies increase under osmotic shock depends on the stress conditions and the presence of Hog1. Wild-type and hog1Δ (PAY185) strains expressing a GFP-tagged version of the decapping enzyme Dcp2p or BY4741 and BY4741hog1Δ strains expressing the GFP-tagged version of the decapping activator Dhh1p were grown until exponential phase and then treated with (A) NaCl 0.4 M or (B) KCl 1 M. Dcp2-GFP and Dhh1-GFP were used to visualize P-bodies at the indicated times of osmotic stress. (C) Quantification of the percentage of wt or hog1Δ cells with P-bodies after treatment with 0.4 M NaCl or 1 M KCl as indicated above. At least 100 cells were quantified in each condition.
