
General mRNA stabilization in hog1Δ mutant after treatment with 0.4 M NaCl. (A) Half-lives of RPS31, URA1, and DSE2 mRNAs in strain PAY532 (rpb1-1ts hog1Δ) and of RTN2 mRNA in strain PAY591 (rpb1-1ts hog1Δ rtn2Δ) containing p416TEF-RTN2 were measured as described in Figure 2. The half-life value of a representative experiment is shown. (B) mRNA half-life change (%) after mild osmotic stress in hog1Δ strain. Means were calculated from at least three independent experiments. Error bars represent the standard deviation.










