Analysis of interactions between rpS4 and rpS5 mutants using β-galactosidase activity. (A) β-Galactosidase assays. Each column represents the percent β-galactosidase activity relative to the wild type. The results are average of at least three independent assays done with three to four transformants each time. The error bars represent standard error of the mean. (B) Western blot to determine the relative levels of protein expressed. Extracts from yeast transformants were made by vortexing with glass beads in lysis buffer as described in Materials and Methods. Twenty micrograms of total protein for each sample were loaded and detected using LexA antibody. Detection with a β-tubulin (asterisk) antibody controlled for loading.
