A single C. elegans PUF protein binds RNA in multiple modes

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FIGURE 7.
FIGURE 7.

Mutational analysis of position -1 in PUF-11 binding sites. (A) Base substitutions at position −1 of three RNAs analyzed using the three-hybrid assay. The core binding element of each RNA is shown; UGU is shaded and the mutated base is underlined. β-galactosidase values are relative to the respective wild-type RNAs. (B) Base substitutions at position −1 of three RNAs analyzed in vitro. Synthetic RNAs with the indicated base substitution at position −1 were analyzed in EMSAs with purified recombinant GST–PUF-11. Krel is the ratio of the Kd relative to the respective wild-type RNAs.

This Article

  1. Published in Advance April 15, 2009, doi: 10.1261/rna.1545309 RNA 15: 1090-1099

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