PUF-11 binds to three classes of RNAs. β-galactosidase activity as determined using strains possessing each of the indicated RNAs. β-galactosidase activity reflects the strength of PUF–RNA interactions (Hook et al. 2005). Values are relative to RNA I-1. The sequence in the relevant region of each bound RNA is indicated and corresponds to the portion that had been randomized. RNAs are named as X-Y, where X indicates the class, and Y indicates relative affinity for PUF-11 within the class. RNAs I-1, II-1, and III-1 were included when testing each class of RNAs, to provide standards. The consensus sequence for each class was derived using WebLogo software and displays information content and frequency of nucleotide occurrence at each position.
