Mammalian GW182 contains multiple Argonaute-binding sites and functions in microRNA-mediated translational repression

(Downloading may take up to 30 seconds. If the slide opens in your browser, select File -> Save As to save it.)

Click on image to view larger version.

FIGURE 5.
FIGURE 5.

Two Ago2-binding sites exist in the amino-terminal half of TNRC6B. (A,B,D) SBP pull-down assays of Ago2 and TNRC6B. HEK293F cells were cotransfected with FLAG/SBP or FLAG-tagged Ago2 and the TNRC6B expression vectors. (C) HEK293F cells were cotransfected with FLAG-Ago2 and FLAG-EGFP-TNRC6B or FLAG-EGFP expression vectors as a negative control. (E,F) HEK293F cells were cotransfected with FLAG-Ago2 and wild-type or mutant FLAG/SBP-TNRC6B expression vectors. SBP-pull down assays and Western blotting were performed as shown in Figure 1C.

This Article

  1. Published in Advance April 27, 2009, doi: 10.1261/rna.1363109 RNA 15: 1078-1089

Current Issue

  1. August 2026, 32 (8)
  1. Current Issue
  1. Alert me to new issues of RNA