Mammalian GW182 contains multiple Argonaute-binding sites and functions in microRNA-mediated translational repression

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FIGURE 4.
FIGURE 4.

GST pull down assay of Ago2 and GST-fused GW182 mutants. (A) The wild-type and mutated GST-GW182 (445–526 and 716–806) fragments were each expressed in E. coli. (B) The wild-type and mutated GST-GW182 (778–910) fragments were each expressed in E. coli. HEK293F cell extracts overexpressing FLAG-Ago2 were incubated with each MagneGST particle-immobilized GST-GW182 fragment. The resulting complexes were resolved by SDS-PAGE, followed by Coomassie Brilliant Blue (CBB) staining (bottom), and were analyzed by Western blotting using an anti-FLAG M2 antibody (top).

This Article

  1. Published in Advance April 27, 2009, doi: 10.1261/rna.1363109 RNA 15: 1078-1089

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