GW182 Q/N-rich region is required for P-body localization. (A–H) GFP-tagged AGO1 or mutants were expressed in S2 cells. In panels C–H, the effect of cotransfecting HA-GW182 or HA-GW182 mutants on the localization of AGO1 was examined. The merged images show the GFP signal in green, the HA signal in red. (I–N) Confocal fluorescent micrographs of fixed S2 cells expressing HA-tagged fusions of full-length GW182 or GW182 mutants. Cells were stained with affinity-purified anti-Tral antibodies. The merged images show the HA signal in green and the anti-Tral signal in red. In all panels, the fraction of cells stained identically to the representative panel was determined by scoring at least 100 cells in two independent transfections performed per protein. Scale bar, 5 μm.
