A C-terminal silencing domain in GW182 is essential for miRNA function

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FIGURE 4.
FIGURE 4.

Mutations in AGO1 that uncouple miRNA and GW182 binding. (A) Lysates from S2 cells expressing HA-tagged versions of MBP, wild-type AGO1, or AGO1 mutants were immunoprecipitated using a monoclonal anti-HA antibody. Inputs (1.5%) and immunoprecipitates (30%) were analyzed by Western blotting using a polyclonal anti-HA antibody. Endogenous GW182 was detected using anti-GW182 antibodies. The association between HA-AGO1 and endogenous bantam was analyzed by Northern blotting. tRNAAla served as a loading control. (Asterisk) IgG cross-reactivity.

This Article

  1. Published in Advance April 21, 2009, doi: 10.1261/rna.1605509 RNA 15: 1067-1077

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