The C-terminal domains of human TNRC6A, TNRC6B, and TNRC6C silence bound transcripts independently of Argonaute proteins

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FIGURE 3.
FIGURE 3.

The AGO-binding domains and the Q-rich regions are required for P-body localization. (A) Localization of HA-TNRC6C in HeLa cells expressing GFP-AGO1–AGO4. (B) Localization of HA-tagged N- or C-terminal fragments of HA-TNRC6A–TNRC6C in HeLa cells expressing GFP-AGO2. (C) Localization of TNRC6 mutants in HeLa cells expressing GFP-AGO2. (D) Localization of HA-TNRC6A, TNRC6B, and TNRC6C lacking the Q-rich regions in HeLa cells expressing GFP-AGO2. In all panels, the merged images show the GFP signal in green and the HA signal in red. The fraction of cells exhibiting a staining identical to that shown in the representative panel was determined by scoring at least 100 cells (showing Argonaute-containing foci) in two independent transfections performed per protein. Scale bar, 10 μm.

This Article

  1. Published in Advance April 21, 2009, doi: 10.1261/rna.1606309 RNA 15: 1059-1066

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