The N-terminal GW-repeat-containing regions of TNRC6A–TNRC6C are required for the interaction with AGO1–AGO4. (A–C) GFP-AGO1–AGO4 and HA-TNRC6A–TNRC6C N- and C-terminal fragments were coexpressed in human HEK293 cells as indicated. Cell lysates were immunoprecipitated using anti-GFP antibodies. GFP-MBP served as a negative control. Inputs and immunoprecipitates were analyzed by Western blot as described in Figure 1, B–E. (D) Wild-type HA-TNRC6C or mutants were coexpressed with GFP-AGO2 or GFP-MBP in human HEK293 cells as indicated. Cell lysates were immunoprecipitated using anti-GFP antibodies. Inputs (2%) and immunoprecipitates (50%) were analyzed as described in A–C.
