Regulation of NAB2 mRNA 3′-end formation requires the core exosome and the Trf4p component of the TRAMP complex

The NAB2 A₂₆ sequence inhibits mRNA 3′-end processing in vitro. Processing of synthetic ³²P-labeled RNAs in vitro. (A) Diagram of the synthetic CYC1 RNA showing the portion (white) 5′ to the cleavage site and (black) 3′ to the cleavage site. (B) Processing of the CYC1 RNA in vitro. (Right) The diagrams show the positions of the substrate and products of the reaction. (Left) The numbers refer to the positions of the length markers (M). (C) Diagram of the synthetic NAB2 RNAs showing the portion (white) 5′ and (black) 3′ to the A₂₆ or U₂₆. (D) Processing of the CYC1 RNA in vitro. (Right) The diagrams show the positions of the substrate and products of the reaction. (Left) The numbers refer to the positions of the length markers (M).