Mutations in CPF and CFIA components cause NAB2 mRNA 3′-end formation defects. (A,B) Northern blot analysis of total RNA isolated from strains (A) YSB213 (WT), YSB215 (pap1-1), or YSB202 (rna14-1); and (B) FY41 (PTA1), FY1284 (pta1-2), YT2 (YTH1), and YT3 (yth1ΔN1) before and after shift for 1 h from 25°C to 35°C. All strains were grown in YPD, except YT2 and YT3, which were grown in synthetic complete dextrose without uracil (Fink 1991). (C) Northern blot analysis of the products of RNase H cleavage of total RNA from strains with the indicated genotypes after a 1-h shift to 35°C. (D) Northern blot analysis of total RNA prepared from strains with the indicated genotypes before (25°C) and after shift for 1 h to 35°C.
