Regulation of NAB2 mRNA 3′-end formation requires the core exosome and the Trf4p component of the TRAMP complex

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FIGURE 2.
FIGURE 2.

The mature 3′-end of NAB2 mRNA maps to the A26 sequence in the 3′-UTR. (A) Agarose gel electrophoretic analysis of the product of LM-PAT analysis of the strains with the indicated genotypes. (M) The lane with molecular size standards. (B) Diagram of the cRT-PCR method for a model mRNA. TAP (tobacco acid pyrophosphatase) treatment removes the cap, leaving a 5′-phosphate and the 3′-poly(A) tail, which is ligated (LIG) with RNA ligase to produce a circular RNA that is amplified by RT-PCR with (arrows) specific primers. The sequences obtained using primers to NAB2 mRNA are shown below the genomic sequence with the start codon (ATG) and A26 sequence in bold.

This Article

  1. Published in Advance April 15, 2009, doi: 10.1261/rna.709609 RNA 15: 1045-1058

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