FIGURE 3.
Analytical ultracentrifugation. (A) Sedimentation velocity analysis showing a plot of c(s) (continuous sedimentation coefficient) distribution versus sedimentation coefficient of human Pum Puf domain-NRE1 and -NRE2 complexes. The sedimentation coefficients have been corrected to standard conditions and are reported in units of Svedbergs, where 1 S = 1 × 10−13 sec. (B) Models of Pum-NRE2 complex. Model 1, one Pum molecule bound to the Box B site; model 2, two Pum molecules modeled head-to-tail on an “extended” NRE2; and model 3, two Pum molecules modeled head-to-tail on “circular” NRE2.
