Tailoring RNA modular units on a common scaffold: A modular ribozyme with a catalytic unit for β-nicotinamide mononucleotide-activated RNA ligation

Structural requirements of the YFL-1 ribozyme. (A) Mutations at the (upper) P2 region and (lower) the loop–receptor interactions between P1 and P3. Mutations were introduced in the P2 region, (red) GAAA loop in P1, and/or (green) receptor motif in P3 in Figure 1D. (B) Activities of the YFL-1 variants with mutations in the P1 loop or P2 region. (C) Activities of the YFL-1 variants with mutations in the P2 region shown in Figure 6A, upper. (D) Activities of the YFL-1 variants with mutations in loop–receptor motifs shown in Figure 6A, lower.