Tailoring RNA modular units on a common scaffold: A modular ribozyme with a catalytic unit for β-nicotinamide mononucleotide-activated RNA ligation

Metal ion dependence of the activity of the YFL-1. (A) Time courses of the ligation reaction by YFL-1 (filled circles) with 200 mM K⁺ and 50 mM Mg²⁺, (filled squares) with 50 mM Mg²⁺ and no K⁺, and (open circles) with 200 mM K⁺ and no Mg²⁺. (B) Reactions of YFL-1 with different divalent metal ions and Co(NH₃)³⁺. Reactions were carried out for 2 h in the presence of 50 mM divalent metal ion or Co(NH₃)³⁺. (C) Time courses of the ligation reaction of YFL-1 with (open circles) 10 mM, (filled squares) 25 mM, (filled circles) 50 mM, (open squares) 100 mM, and (open triangles) 150 mM Mg²⁺ ions and 200 mM KCl.