Tailoring RNA modular units on a common scaffold: A modular ribozyme with a catalytic unit for β-nicotinamide mononucleotide-activated RNA ligation

Activity of the YFL-1 ribozyme primed by the reduced form of β-NMN. (A) Time course of the reaction between 1 μM YFL-1 ribozyme and 1 μM 5′-FAM-labeled substrate. The reaction was performed with 30 mM Tris-HCl (pH 7.5), 50 mM MgCl₂, and 200 mM KCl at 37°C. (B) Activity of the YFL-1 ribozyme. Reactions were carried out with (filled circles) equivalent amounts of ribozyme and substrate or (open squares) an excess of substrate. (C) Reactions of YFL-1 with modified substrates. The modified substrate had 2′-methoxycytidine, 2′-deoxycytidine, or 3′-deoxycytidine at the 3′-terminus. Reactions were carried out for 6 h.