Polyadenylation releases mRNA from RNA polymerase II in a process that is licensed by splicing

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FIGURE 4.
FIGURE 4.

Polyadenylation releases RNA whose only attachment to the polymerase is through the CPA. Pull-down Method 1 was used. A construct with a mutant 3′ splice site (AG → ct) was used. The RNA in lanes 3 and 4 was oligo(dT) selected prior to loading on the gel.

This Article

  1. Published in Advance March 20, 2009, doi: 10.1261/rna.1409209 RNA 15: 823-836

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