FIGURE 2.
GW182 fragment GW1Δ10 (“aa566-1343”) recruited Ago2 to cytoplasmic foci by interacting with the C-terminal half of Ago2. GFP-GW1Δ10 (green, a–c) was cotransfected with Flag-Ago2 (d), PIWI (“aa478-860,” f) or PAZ (“aa1-480,” h) into HeLa cells. As controls, Flag-PIWI (e) or Flag-PAZ (g) was singly transfected. The cells were stained with anti-Flag antibody (red, d–h). Panels in the bottom row are the merged images (i–m). Nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI) (blue). Scale bar, 10 μM.
