FIGURE 3.
Repression mediated by dGW182 and its sufficiency domains is independent of poly(A) tail. Drosophila S2 cells were transfected with a boxB-containing firefly luciferase reporter, either (open bars) FLuc-boxB or (black bars) FLuc-boxB-HSL. Unlike FLuc-boxB, FLuc-boxB-HSL lacks polyadenylation signal and bears histone H1 stem–loop structure in its 3′-UTR. Together with a firefly luciferase reporter, RLuc as a normalization control and one of the NHA-fusion proteins (NHA-dGW182, or NHA-dGW182 deletion mutant, or NHA-lacZ as a negative control) were co-transfected. For the rest, the assay was performed as described in Figure 1.
