Real-time RT-PCR validation of the miRNA assays. (A) The assay specificity was evaluated with two synthetic miRNAs, miR-124a and let-7a. Five miRNA assays were evaluated for the detection of these synthetic miRNAs. The assay readings of miR-124a by miRNA-124a assay, and let-7a by let-7a assay were used as the relative standards (100%) for the analysis of assay cross-reactivity. (B) The assay specificity was evaluated with two cell lines and 96 cancer-related miRNA assays. One cell line is SKOV-3 and the other one is SKOV-3-miR-146a, which is SKOV-3 with stably expressed miR-146a. Each data point represents the normalized readings from one miRNA assay in both cell lines.
