FIGURE 1.
The experimental strategy for a new miRNA expression profiling platform with real-time RT-PCR. Multiplexed RT is performed with a pool of miRNA-specific RT primers. These RT primers contain unique tag sequences at the 5′-end. Real-time PCR is then performed with the cDNA templates generated from the multiplexed RT reaction. The PCR reverse primer specifically anneals to the 3′-end of the cDNA template, and the PCR forward primer specifically anneals to the tag sequence in the RT primer.
