Expression of MT-COMP mRNA is decreased in a dose-dependent manner in primary hCCCs infected with increasing MOIs of adenovirus expressing Ribo56. Normal costochondral chondrocytes were isolated as previously described and were transfected in monolayer with an increasing MOI of AdRibo56 (shown above the gel). RNA was collected after 96 h. Total RNA (7 μg) was subjected to Northern analysis using radiolabeled probes specific for COMP and human GAPDH (loading control). Representative autoradiographs (N = 5) are shown above, and COMP expression in uninfected cells was used to normalize to 1. Error bars represent the SEM, and P < 0.05 was considered significant (*).
