Adenovirus-based COMP expression is increased in a dose-dependent manner in COS7 cells when coinfected with adenovirus expressing Ribo56. COS7 cells were coinfected with either AdWT- or AdMT-COMP with an increasing MOI of AdRibo56. RNA and cellular proteins were collected after 96 h. (A) Total RNA (7 μg) was subjected to Northern analysis using radiolabeled probes specific for COMP and human GAPDH (loading control). Resulting autoradiographs are shown above, while normalization of COMP mRNA knockdown is displayed in the bar graph. COS7 cells expressing only COMP (0×) were normalized to 1. (B) Protein (30 μg) was subjected to Western analysis using antibodies specific for COMP and β-actin (loading control). Shown are the resulting autoradiographs and normalized data.
