Potency of Alexa555-labeled siLAM and siLAM-peptide conjugates after lipofectamine-mediated delivery. Dose–response curves and IC50 values of inhibition of lamin A/C expression by (A) Alexa555-siLAM-pTOX, (B) Alexa555-siLAM-gTOX, or (C) Alexa555-siLAM after transfection. ECV-304 cells were treated with the indicated concentrations of Alexa555-siLAM-pTOX, Alexa555-siLAM-gTOX, or Alexa555-siLAM using lipofectamine as transfectant. After 24 h in culture, cells were collected, their total RNA was extracted and reverse transcribed, and lamin A/C mRNA levels were determined by quantitative PCR. (g) Guide strand; (p) passenger strand; (TOX) TQIENLKEKG.
