FIGURE 5.
Stability of siLAM and siLAM-gTOX. Both siRNAs (f.c. 0.5 μM) were incubated in (A) medium 199 containing 10% (v/v) FCS or (B) cytosolic extracts (S100 fraction) at 37°C. Aliquots withdrawn at indicated time points were rapidly frozen using liquid nitrogen and stored at −80°C. Samples were analyzed on a 20% (w/v) acrylamide nondenaturing PAGE, and RNA was detected using a PhosphorImager after staining with Sybr Gold dye. (g) guide strand; (TOX) TQIENLKEKG.
