Potency of siLAM and siLAM–peptide conjugates after lipofectamine-mediated delivery. Dose–response curves and IC50 values of inhibition of lamin A/C expression by (A) siLAM-pTOX, (B) siLAM-gTOX, or (C) siLAM after transfection. ECV-304 cells were treated with the indicated concentrations of siLAM, siLAM-gTOX, or siLAM-pTOX using lipofectamine as transfectant. After 24 h in culture, cells were collected, their total RNA was extracted and reverse transcribed, and lamin A/C mRNA levels were determined by quantitative PCR. (D) Sequence-specificity of lamin A/C mRNA suppression by siLAM and siLAM-pTOX. ECV-304 cells were transfected with 1 nM of siLAM or siLAM-pTOX using lipofectamine. Lamin-A/C mRNA levels were determined as described. (g) Guide strand; (p) passenger strand; (mut) G → C transversion at positions 10 and 11 of the guide strand; (TOX) TQIENLKEKG.
