The linear form of a group II intron catalyzes efficient autocatalytic reverse splicing, establishing a potential for mobility

(Downloading may take up to 30 seconds. If the slide opens in your browser, select File -> Save As to save it.)

Click on image to view larger version.

FIGURE 3.
FIGURE 3.

Reverse-splicing precision. Reverse-splicing product was isolated and the 3′-splice site was sequenced by primer extension (right). Control experiments sequencing the splice sites of substrate RS3 (left) and of a linear ai5γ transcript with flanking exons bearing the expected splice site junction (center) were performed with the same primer.

This Article

  1. Published in Advance January 23, 2009, doi: 10.1261/rna.1392009 RNA 15: 473-482

Current Issue

  1. July 2026, 32 (7)
  1. Current Issue
  1. Alert me to new issues of RNA