Characterization of a heat-stable enzyme possessing GTP-dependent RNA ligase activity from a hyperthermophilic archaeon, Pyrococcus furiosus

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FIGURE 4.
FIGURE 4.

Time course of forward and reverse RNA ligation reaction of PF0027 protein. (A,B) Two different substrates bearing 2′,3′-cyclic phosphate and 5′-OH termini were used for the ligation reaction. The reaction was carried out with 1 μg/mL purified PF0027 protein at 37°C in the presence of 1 mM GTP.

This Article

  1. Published in Advance January 20, 2009, doi: 10.1261/rna.1122109 RNA 15: 420-431

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