Involvement of SSU-processome components in rRNA synthesis. (A) rDNA unit and probes used in the TRO analysis, as described in Figure 3. (B) Residual level of expression of targeted Utps (Utp5, Utp7, and Utp15) in GAL-regulated strains expressing functional amino-terminal HA epitope tagged constructs. Yeast cells were grown to mid-log phase in galactose medium and transferred to glucose for the indicated time points. Equivalent amounts of total protein extracts were analyzed by α-HA Western blotting. PGK was used as a loading control. Residual level of expression of Utp5 and Utp15 were quantitated with ImageQuant (GE Healthcare). (C) TRO analysis was conducted as described in Figure 3. The experiments were performed in triplicate, and representative slot blots are shown. (D) PhosphorImager quantitation. Signal was normalized to 5S.
