Investigating the specificity and stoichiometry of RNA binding by the nucleocapsid protein of Bunyamwera virus

Analysis of RNA species bound to BUNV N protein tetramers. (A) RNA was harvested from purified tetramers and subjected to electrophoresis on a 10% denaturing polyacrylamide gel, alongside single strand RNA size markers. Lanes 1 and 2 are duplicate lanes, and lane 3 shows reduced loading. (B) N/RNA tetramers were incubated with increasing quantities of RNase A, after which the RNA was extracted and electrophoresed on a 1.5% nondenaturing agarose gel alongside single strand RNA size markers and undigested RNA.