Comparison of size and concentration-dependent silencing in C. elegans and Drosophila cells. Size and concentration-dependent silencing triggered by addition of dsRNA to the growth medium of cultured C. elegans and Drosophila cells expressing wild-type or mutant SID-1. (A,B) Cultured C. elegans cells expressing myo-3∷luciferase∷GFP were exposed to the indicated concentration of 500 bp (A) and indicated lengths (B) of luciferase dsRNA and luciferase levels measured 48 h later. The qt9 allele is an early stop codon mutant (Winston et al. 2002). (C,D) Drosophila S2 cells transiently transfected with wild-type SID-1 or missense mutant SID-1(S536I) constructs were exposed to the indicated concentration of 500 bp (C) and indicated lengths (D) of luciferase dsRNA and luciferase levels measured 24 h later. Error bars = 1 standard deviation, n = 3.
