FIGURE 2.
P-FMRP associates with increased amount of precursor miRNAs. (A) We immunoprecipitated 2 × 109 Flag-FMRP cells with pre-immune serum (pre), NP, or PSER. RNA was extracted and end-labeled with P32 as described (Duan and Jin 2006), resolved on a 15% acrylamide TBE/urea gel, and visualized using autoradiography (8 h). The longer exposure of the gel (16 h, right) shows associated miRNAs. Decade marker system (Ambion) indicates RNA size in nucleotides. (B) Equivalent amounts of FMRP were immunoprecipitated by PSER and NP antibodies in A.
