Translation initiation complex formation in the crenarchaeon Sulfolobus solfataricus

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FIGURE 2.
FIGURE 2.

Discrimination by aIF1 against a non-AUG start codon. (A) AUG-mRNAF binding to 30S•aIF1A•a/eIF2565-GTP•Met-tRNAiMet in the absence (green trace) and presence (red trace) of aIF1. AUU-mRNAF binding to 30S•aIF1A•a/eIF2565-GTP•Met-tRNAiMet in the absence (blue trace) and presence (orange trace) of aIF1. Control measurements were performed with unlabeled AUG-mRNA and labeled a/eIF2565 (black trace, acceptor only) or with labeled AUG-mRNAF and unlabeled a/eIF2 (gray trace, donor only). (B) AUG-mRNAF binding to 30S•aIF1A•a/eIF2565-GTP in the absence of Met-tRNAiMet and in the presence (red trace) or absence (green trace) of aIF1. AUU-mRNAF binding to 30S•aIF1A•a/eIF2565-GTP in the absence (blue trace) and presence (orange trace) of aIF1. The charts in A and B show the kav values determined as described in Materials and Methods.

This Article

  1. Published in Advance October 27, 2009, doi: 10.1261/rna.1662609 RNA 15: 2288-2298

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