FIGURE 1.
Hydroxyl radical probing of 16S rRNA in 30S/aIF1 complexes. (A,B) Primer extension analysis of helices 23 (A) and 24 (B) in 16S rRNA in 30S/aIF1 complexes upon hydroxyl radical probing. The individual lanes are indicated: 16S rRNA sequencing (T, A, C, and G), 30S modified in the absence of the factor (30S) or in the presence of the stoichiometric excess of aIF1 (2×, 5×, and 10×, as indicated). (C,D) Elements of helices 23 and 24 of 16S rRNA with protected sites shown by bars.
