Characterization of the functional role of nucleotides within the URE2 IRES element and the requirements for eIF2A-mediated repression

(Downloading may take up to 30 seconds. If the slide opens in your browser, select File -> Save As to save it.)

Click on image to view larger version.

FIGURE 8.
FIGURE 8.

Eukaryotic initiation factor 2A-mediated repression is not specific to the URE2 IRES element. Using the p281-4 reporter plasmid (Altmann et al. 1993), the activity of the invasive growth IRES elements (Gilbert et al. 2007) was tested in both wild-type (gray bars) and ΔeIF2A (black bars) yeast cells. IRES activity is represented in Miller units as in Figure 2.

This Article

  1. Published in Advance October 27, 2009, doi: 10.1261/rna.1722809 RNA 15: 2264-2277

Current Issue

  1. August 2026, 32 (8)
  1. Current Issue
  1. Alert me to new issues of RNA