In vivo detection of RNA-binding protein interactions with cognate RNA sequences by fluorescence resonance energy transfer

Validation of vector expression utilized in this study. (A) Schematic representation of the protein expression vectors utilized in the study. (B) Schematic representation of the constructs generating RNA substrates utilized in the study. (C) Expression of the key fusion proteins used in the study. HeLa cells were transfected by the indicated expression plasmid (top). The total cell lysates were analyzed by Western blot with the indicated antibody. Identity of the various fusion proteins is indicated on the right. Endogenous hnRNP H is also marked.