Real-time fluorescence detection of exoribonucleases

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FIGURE 7.
FIGURE 7.

Correlation between relative fluorescence units and concentrations of released FAM-labeled nucleotides. A linear correlation between fluorescence units and digested RNA concentrations was determined. The increase of fluorescence signal (in relative units) reflect, therefore, directly and linearily the increase in total RNA digestion in molarity (see Materials and Methods).

This Article

  1. Published in Advance September 18, 2009, doi: 10.1261/rna.1670909 RNA 15: 2057-2062

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