Real-time fluorescence-based analysis of RNA hydrolysis by an exoribonuclease under various conditions. The experiment was performed in similar conditions to those in Figure 1. (A) Detection of an inhibitor of Xrn1 activity. Kinetics of RNA hydrolysis by Xrn1 alone or in the presence of ATP, ADP, AMP, or pAp. A specific inhibition of Xrn1 by pAp can be detected, as has been previously published (Dichtl et al. 1997). (B) Detection of protein complex activity. Hydrolysis of 500 nM of RNA1 by Rat1 of S. pombe alone (150 nM) or in the presence of Rai1 (150 nM) (Xiang et al. 2009). RT-FeDEx analysis shows the increased activity of Rat1 in the Rat1–Rai1 complex, as has been reported (Xue et al. 2000, 2009).
