The ionic environment determines ribozyme cleavage rate by modulation of nucleobase pKa

(Downloading may take up to 30 seconds. If the slide opens in your browser, select File -> Save As to save it.)

Click on image to view larger version.

FIGURE 3.
FIGURE 3.

Rate versus pH curves for RG in NH4 + or Mn2+. The apparent first-order cleavage rate constant, k obs, of the RG version of the VS ribozyme was determined in the presence of (A) 3.75 M NH4Cl and (B) 25 mM MnCl2. Data in NH4Cl were fit to Equation 3 and data in MnCl2 were fit to Equation 4 (solid lines), as described in Materials and Methods. Parameter values are presented in Table 1.

This Article

  1. Published in Advance August 12, 2008, doi: 10.1261/rna.1102308 RNA 14: 1942-1949

Current Issue

  1. August 2026, 32 (8)
  1. Current Issue
  1. Alert me to new issues of RNA