FIGURE 5.
Comparable shRNA- and miRNA-based vectors exhibit appropriate strand biasing. (A–C) Strand biasing of SCA1, HD, and GFP RNAi vectors, respectively. Strand biasing was assessed using luciferase reporters containing either sense (intended) or antisense (unintended) target sequences. RNAi luciferase reporter and RNAi expression plasmids were cotransfected into HEK293 cells, and Dual-Glo Luciferase assays were performed at 24 h. Results of duplicate experiments (each n = 3) are shown as mean±SEM relative to mock-treated controls.
