Minimizing variables among hairpin-based RNAi vectors reveals the potency of shRNAs

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FIGURE 4.
FIGURE 4.

Design of comparable shRNA and artificial miRNA hairpins. Diagram depicting the design of hairpins for a fair comparison scheme. Relevant cleavage sites mapped by 3′-RACE are shown (Table 1). Vectors were designed to contain siRNAs targeting SCA1, HD, or GFP transcripts (Ns).

This Article

  1. Published in Advance August 12, 2008, doi: 10.1261/rna.1062908 RNA 14: 1834-1844

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