(A) Vigilin associates with SUV39H1 and HP1α in vivo. HEK293 nuclear lysates were used for immunoprecipitation with the indicated antibodies. Western blotting was with anti-vigilin antibody and anti-Ku70 antibody. (B) SUV39H1 interacts with the C-terminal domain of vigilin. HEK293 cells were transfected with N-terminal Flag-tagged vigilin. Seventy-two hours after transfection, nuclear lysates were used for immunoprecipitation with anti-SUV39H1 antibody. Western blotting was with anti-flag antibody. (C) Seventy-two hours after transfection of HEK293 cells with GFP-tagged vigilin constructs, whole-cell lysates were used for Western blotting with anti-GFP antibody. Equal amounts of extracts were examined, showing that all fragments were expressed at comparable levels. (D) Full-length vigilin and mutant constructs.
