Pyrrolo-C as a molecular probe for monitoring conformations of the tRNA 3′ end

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FIGURE 4.
FIGURE 4.

Aminoacylation of tRNA by E. coli CysRS. (A) Aminoacylation plateau of 1 μM of tRNA-CCA and tRNA-CPyCA with 35S-cysteine catalyzed by 4 μM of CysRS. The percent of aminoacylation was based on the amount of synthesized 35S-cysteinyl-tRNACys versus that of the input tRNA as determined from absorption at 260 nm (1 OD260 = 40 μg/mL). (B) Aminoacylation kinetics of 1 μM of tRNA-A76, tRNA-CCA, and tRNA-CPyCA (as purified from Fig. 2B) with 35S-cysteine catalyzed by 1 nM of CysRS. The concentration of tRNA was determined from absorption at 260 nm while that of CysRS was determined from the Bradford assay and corrected by active site titration.

This Article

  1. Published in Advance August 28, 2008, doi: 10.1261/rna.1158508 RNA 14: 2245-2253

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