Pyrrolo-C as a molecular probe for monitoring conformations of the tRNA 3′ end

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FIGURE 3.
FIGURE 3.

Liquid chromatography/mass spectrometry analysis of the E. coli tRNACys-CPyCA transcript digest to single nucleosides. (A) RP-chromatogram on C18 showing the UV trace at 260 nm and the percent (v/v) of acetonitrile. The commercially available PyCTP (Glen Research) digested to the nucleoside form was used to provide the marker for PyC with the retention time of 20.5 min. (B) Mass spectrometry of the PyC peak isolated from A, showing the m/z value of 282.1 for the protonated nucleoside of PyC and m/z value of 150.1 for the protonated PyC base.

This Article

  1. Published in Advance August 28, 2008, doi: 10.1261/rna.1158508 RNA 14: 2245-2253

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