Pyrrolo-C as a molecular probe for monitoring conformations of the tRNA 3′ end

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FIGURE 1.
FIGURE 1.

PyC as a fluorescence probe in E. coli tRNACys. (A) The chemical structure of PyCTP used for incorporation by Bst CCA-adding enzyme. Inward and outward arrows denote hydrogen bond acceptors and donors, respectively. (B) A scheme showing the cloverleaf of E. coli tRNACys-C74 as the substrate for Bst CCA enzyme for incorporation of PyC75 and A76, using PyCTP and ATP as the substrates.

This Article

  1. Published in Advance August 28, 2008, doi: 10.1261/rna.1158508 RNA 14: 2245-2253

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