Long-distance placement of substrate RNA by H/ACA proteins

Thermodynamic parameters of substrate RNA docking. (A) The apparent dissociation constant of L7Ae, K_d (L7), was obtained by monitoring florescence intensities as a function of L7Ae concentration followed by nonlinear least-square fitting. In the titration experiment, the CNG complex was in molar excess of the guide–substrate RNA complex. (B) The apparent dissociation constant of CNG, K_d (CNG), was obtained by monitoring florescence intensities as a function of CNG concentration followed by nonlinear least-square fitting. In the titration experiment, L7Ae was in molar excess of the guide–substrate RNA complex.